Figure 2.
Silent mating‐type information regulation 2 homolog 1 (SIRT1) inhibition suppresses proliferation of adapted suspension cells (ASC). A, Adherent cells (AD) and ASC were plated at a density of 5 × 105 per well onto six‐well plates and cultured for 72 h with the indicated concentrations of nicotinamide (NAM). Number of cells was counted, respectively. B, Number of cells was counted at the indicted time points after treatment with 1 mmol/L NAM. C, Cells transfected with control siRNA or SIRT1 siRNA (40 ng/mL) were cultured for 48 h, and the efficiency of siSIRT was examined by immunoblot assay. D, Number of cells treated with siSIRT1 or siControl was counted 72 h after the treatment. *P < .05, **P < .01; n.s., not significant