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. 2019 Aug 29;10:1027. doi: 10.3389/fpls.2019.01027

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Copyright © 2019 Wang, Liu, Gao, Rui and Tang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of AvrPtoB leads to stunted phenotypes in Arabidopsis. (A) Five-week-old plants of Col-0, the exo70B1-3 transgenic line (expressing EXO70B1-GFP in the exo70B1-3 mutant background), and two independent 35S:AvrPtoB-Myc transgenic Arabidopsis plants in the background of the exo70B1-3 transgenic line were photographed under short-day conditions. Bar = 1.0 cm. (B–E) RT-qPCR analysis of TN2 (B), PR1 (C), PR2 (D), and immune-related genes (E) expression. Total RNA was isolated from leaves of the 5-week-old Arabidopsis plants indicated in (A). The expression levels of indicated gene were normalized to the reference gene ACTIN2. Data represent the mean and standard deviation of three biological replicates. Three technical replicates for each biological sample were used. The lowercase letters indicate statistically significant differences (P < 0.05; one-way ANOVA). These experiments were repeated three times with similar results.