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. 2019 Aug 29;10:1027. doi: 10.3389/fpls.2019.01027

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Copyright © 2019 Wang, Liu, Gao, Rui and Tang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The stunted phenotypes of the AvrPtoB overexpression lines are partially dependent on TN2. (A) Five-week-old Col-0, tn2-10, and two independent 35S:AvrPtoB-Myc transgenic Arabidopsis plants (35S:AvrPtoB-Myc #2 and 35S:AvrPtoB-Myc #7) in the Col-0 and tn2-10 background were photographed under short-day conditions. Bar = 1.0 cm. (B) The AvrPtoB-Myc fusion protein was examined by immunoblotting. Ponceau S staining of Rubisco was shown as a protein loading control. (C–H) RT-qPCR analysis of TN2 (C), PR1 (D), PR2 (E), PAD4 (F), NPR1 (G), and EDS5 (H) expression. Total RNA was isolated from leaves of the indicated 5-week-old Arabidopsis plants. The expression levels of indicated gene were normalized to the reference gene ACTIN2. Data represent the mean and standard deviation of three biological replicates. Three technical replicates for each biological sample were used. The lowercase letters indicate statistically significant differences (P < 0.05; one-way ANOVA). These experiments were repeated three times with similar results.