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. 2019 Aug 29;10:1027. doi: 10.3389/fpls.2019.01027

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Copyright © 2019 Wang, Liu, Gao, Rui and Tang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EXO70B1 suppresses TN2- or TN2-TIR-triggered cell death in N. tabacum. (A) and (B) TN2- or TN2-TIR-triggered cell death was suppressed by EXO70B1, but not EXO70B2, in N. tabacum. (C) and (D) AvrPtoB rescues TN2- or TN2-TIR-triggered cell death, which is suppressed by EXO70B1, in N. tabacum. Agrobacterium GV3101 cells carrying different constructs were mixed prior to adding infiltration buffer. The concentration of each agrobacteria in the mix was brought to OD₆₀₀ = 0.5 and infiltrated into 5-week-old N. tabacum leaves for transient expression. Cell death was observed 36 to 48 h later. Pictures were taken 4 days postinfiltration. These experiments were repeated three times with similar results.