Fig. 3.

summary of the surface marker expression in the different cell fractions. Expression of characteristic ASCs-antigens measured in the stromal vascular fraction before Ficoll density centrifugation, after density centrifugation (SVF), after short-term incubation (Oxacells HP) and after cultivation at passage 0. The staining of the positive marker (A) CD13 CD44, CD73, CD90, CD105, he unstable progenitor marker CD34 (A) and the negative marker (B) CD31, CD45, CD235a and HLA II (HLA-DR, DP, DQ) were performed as single and multiple staining. Significant differences in marker expression between the different groups are marked (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P ≤ 0.0001). Data represents mean expression (percentage) ± SD of all donors.