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. 2019 Sep 4;9:12788. doi: 10.1038/s41598-019-49163-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Linker histone affects both DNA wrapping length and opening angle. Top: Scatter plots of the opening angle θ and protruding DNA arm contour length of wt nucleosomes (a) without (n_wt = 444, black) and (b) with histone H1 (n_{wt +H1} = 445, red); centroids are highlighted in blue. Scatter plots for mutated nucleosomes are shown in Supplementary Fig. S8. The histograms show the normalized probabilities for the opening angle (30° bin width) and the protruding DNA arm contour length (bin width 10 nm). Histone H1 induced a decreased protruding DNA length together with a preference of a distinct opening angle. Bottom: The protruding DNA arm contour length was converted into the wrapping length for (c) wt nucleosome objects and (d) mutated nucleosome objects without and with histone H1 (n_wt = 444, n_{wt +H1} = 445, n_mut = 444, n_{mut +H1} = 448). Normalized histograms of wrapping length are plotted from 60 bp–225 bp (bin width 15 bp). Bars of samples with linker histone H1 are indicated by red edges. DNA wrapping is lower for mutated nucleosomes than wt, but increased in the presence of histone H1 in both cases.