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. 2019 Jul 25;47(8):3886–3897. doi: 10.1177/0300060519857839

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© The Author(s) 2019

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Insulin-like growth factor-1 (IGF-1) reduces myocardial cell apoptosis through activation of the PI3K/Akt pathway. (a) Representative images of immunofluorescent TUNEL staining (green color) and 4, 6-diamino-2-phenylindole staining (DAPI, blue color) taken with a fluorescence microscope (magnification, 400×). (b) Apoptotic index, expressed as the proportion of apoptotic myocytes relative to the total number of myocytes. Data are presented as means ± standard deviations (n = 4). **P < 0.05 vs. I/R group; &P < 0.05 vs. L-IGF-1 + I/R group; #P < 0.05 vs. H-IGF-1 + I/R group; NS, not significant.