Figure 2.

Promoting EV-A71 Replication by Ectopic Expression of Pim1

(A and B) After ectopic expression of Pim1, viral titer was determined following the below-mentioned methods in (A) RD and (B) HeLa cells.

(C and E) Pim1 was ectopically expressed in EV-A71-infected cells at MOI 1 for 24 h in (C) RD and (E) HeLa cells. Protein levels of Pim1 and viral protein VP1 were detected. β-Actin was used as the internal control.

(D) The quantification results of (C).

(F) The quantification results of (E).

(G) siRNA targeting Pim1 3′ UTR at 40 nM was co-transfected with the Pim1 expression plasmid for 48 h in RD cells and then infected with EV71 at MOI 1 for 24 h.

(H) The quantification results of (G). The cell lysates were collected for western blot assay. Protein levels were detected and quantitated. The density of individual protein to β-actin in the control was set as 1, and the relative fold change is indicated.

Data are represented as mean ± SD (n = 3). *p < 0.05, compared with mock group; **p < 0.01, compared with mock group.