Figure 3.
Enhancing Viral IRES Activity by Pim1
(A) A diagram of the dicistronic reporter plasmid, which was used to evaluate EV-A71 IRES activity when Pim1 was modulated.
(B and C) (B) The relative IRES activity after ectopic expression of Pim1 or (C) knockdown of Pim1 in 293T cells.
(D) After co-transfection of the reporter plasmid and individual viral protein expression plasmid into 293T cells for 48 h, the RLuc and FLuc activities were analyzed.
(E) The relative mRNA level of Pim1 was determined by qRT-PCR assay after transfection of the individual viral protein expression plasmid into 293T cells for 48 h.
(F) IRES activities after ectopic expression of 2Apro, with or without silencing of Pim1. Results are represented as mean ± SD from three independent experiments. *p < 0.05, **p < 0.01.