Figure 6.

Inducing AUF1 Cytoplasmic Accumulation by CX-6258

(A) RD cells were treated with CX-6258 at the indicated concentrations for 2 h, and then the cytoplasmic and nuclear proteins were extracted. The cytoplasm and nuclear distribution of AUF1, Sam68, hnRNP K and FUBP1 were determined by western blot assay.

(B) The quantification results of (A).

(C) Pim1-specific siRNAs were transfected into RD cells for 48 h. The cytoplasmic and nuclear proteins were extracted. The protein levels of AUF1, Sam68, hnRNP K, and FUBP1 were examined by western blot assays.

(D) The quantification results of (C).

(E) The cytoplasmic and nuclear distribution of AUF1 was detected in RD cells after treating with CX-6258 at indicated concentrations for 2 h under a fluorescence microscope. The yellow arrows indicated the representative cells of AUF1 translocated in the cytosole.

Results are represented as mean ± SD from three independent experiments. *p < 0.05, **p < 0.01.