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. 2019 Aug 1;20(9):e48084. doi: 10.15252/embr.201948084

Figure EV4. Nuclear flattening is sufficient to activate c‐Jun and YAP independently of actomyosin contractility.

Figure EV4

  1. Representative cells stained for p‐Jun Ser73 (magenta) and for nucleus (cyan) in Ctrl, Blebb, and Blebb + AP conditions. Scale bar = 10 μm. Nuclear heights were measured using Hoechst staining.
  2. Corresponding quantifications of p‐Jun Ser73 nuclear intensity. Data are presented as mean ± s.e.m. (n = 58 minimum from two independent experiments, ****P < 0.001 one‐way ANOVA—Tukey's multiple comparisons post‐test).
  3. Quantifications of YAP activity (nucleo‐cytoplasmic ratio) and p‐Jun Ser63 nuclear intensity in Ctrl and Ctrl + AP conditions. Data are presented as mean ± s.e.m. (n = 25 minimum, ns: not significant, t‐test).
  4. Representative cells stained for p‐Jun Ser63 (magenta) and for DNA (cyan) in Ctrl, Y27632, and Y27632 + AP conditions. Scale bar = 10 μm.
  5. Immunoblots of SUN1, SUN2, and GADPH for MRC5 cells depleted or not for SUN1, SUN2, and SUN1 and SUN2 using siRNA or shRNA approaches.
  6. Quantifications of YAP activity (nucleo‐cytoplasmic ratio) and p‐Jun Ser63 nuclear intensity in cells depleted or not for SUN1 and SUN2. Data are presented as mean ± s.e.m. (n = 20 minimum, t‐test *P < 0.5).
  7. Nucleus heights were measured using Hoechst staining in the indicated conditions. Data are presented as mean ± s.e.m. (n = 18 minimum, ****P < 0.001 one‐way ANOVA—Tukey's multiple comparisons post‐test).