Figure 4. Myc regulates all steps of the RNAPII transcriptional cycle.

• A
  Scheme of the different steps of RNAPII regulation for which the corresponding rates were modeled 34.
• B, C
  Boxplots reporting LPS‐induced changes (log₂FC) for each of the four RNAPII kinetic rates in c‐myc ^wt/wt (wt) and c‐myc ^∆/∆ cells for Myc‐dependent (B) and Myc‐independent (C) induced and repressed genes. P‐values obtained with two‐sample Wilcoxon tests for the comparison between c‐myc ^wt/wt and c‐myc ^∆/∆ cells are reported. The RNAPII ChIP‐Seq time‐course was performed on two biological replicates (each on pools of cells from > 10 mice), and reads were merged after sequencing. All boxplots are as defined in Fig 2I.
• D, E
  Heatmaps showing the variations in RNAPII kinetic rates (log₂FC) on Myc‐dependent (D) and Myc‐independent (E) genes during the LPS time‐course. The gray scale represents the starting level for each parameter in unstimulated cells. The first column on the left reports the changes in Myc share for the same genes. Genes were grouped using hierarchical clustering.
• F
  Boxplots reporting LPS‐induced changes (log₂FC) for each of the four RNAPII kinetic rates in c‐myc ^wt/wt (wt) and c‐myc ^∆/∆ cells for genes of clusters 1 and 9. P‐values obtained with two‐sample Wilcoxon tests for the comparison between c‐myc ^wt/wt and c‐myc ^∆/∆ cells are reported.
• G
  Changes in Myc share in LPS‐treated versus LPS‐untreated cells for the different clusters of panel d. P‐values obtained with two‐sample Wilcoxon tests for the comparison between c‐myc ^wt/wt and c‐myc ^∆/∆ cells are reported.