Figure EV4. S‐HAD treatment does not significantly increase the population percentage of rounded, detached cells.

1. Wild‐type MEF cultures were treated with vehicle (control), S‐HAD (10 μM, 24 h), or cisplatin (CP, 30 μM, 16 h), and the percent of the cells still adhered to the dish counted. Results are shown from three independent cultures, error bars indicate SD, n.s. = not statistically different; ***P < 0.001 as calculated by Student's t‐test.
2. Cyclin C is required for S‐HAD‐enhanced apoptosis. Apoptotic efficiency was measured using Annexin V staining in Ccnc ^−/− MEF cultures treated with cisplatin (30 μM, 24 h) with and without 4 h S‐HAD addition (n = 3). Error bars indicate SD. The percentage of Annexin V‐positive cells in the cisplatin alone treated culture was set to one.