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. 2019 Jul 25;20(9):e47498. doi: 10.15252/embr.201847498

Figure 6. EIF1 and EIF5 modulate RAN translation by determining AUG start codon specificity.

Figure 6

  1. The expression of plasmid‐based NL‐3xF reporters in HEK293 cells with and without over‐expression of EIF1 (two‐way ANOVA with Sidak's multiple comparisons test; n = 9–12/condition). Black asterisks refer to comparisons between empty vector‐transfected and EIF1‐transfected cells; green asterisks refer to comparisons between EIF1‐transfected cells expressing AUG‐NL‐3xF and those expressing a different reporter.
  2. The expression of plasmid‐based NL‐3xF reporters in HEK293 cells with and without over‐expression of EIF5 (two‐way ANOVA with Sidak's multiple comparisons test; n = 9–12/condition). Black asterisks refer to comparisons between empty vector‐transfected and EIF5‐transfected cells; pink asterisks refer to comparisons between EIF5‐transfected cells expressing AUG‐NL‐3xF and those expressing a different reporter.
Data information: For all panels, ***P ≤ 0.001, ****P ≤ 0.0001 for the specified statistical test. Bars represent mean ± SEM (compiled from ≥ 3 replicates).