Table 1.
Antibodies used in this study
| Antigen | Source | Cat. Number | Dilution | Used in Figure | Specificity Ref. |
|---|---|---|---|---|---|
| CK1α | Bethyl | A301‐991A | WB: 1:1,000 | 1D E, G, H and I, and 2D and 4B–D and 5F and EV3A and B, Appendix Fig S1A | 12 |
| CK1α | DSTT (University of Dundee) | Sheep SA527, third bleed | WB: 1:1,000; IF: 1:1,000 | 3B and F, and 4G and I, and 5B and C, and EV4A–C | This study |
| CK1α | Santa Cruz | sc‐6477 | IF: 1:100 | 2A, B and G, and 3D and 5D and EV3C | 12 |
| CK1ε | CST | 12448 | WB: 1:1,000 | 1D and EV5B | 12 |
| CK1ε | DSTT (University of Dundee) | Sheep SA610, second bleed | WB: 1:1,000 | EV4A–C | This study |
| CK1ε | Sigma‐Aldrich | HPA026288 | IF: 1:500 | EV2A | 12 |
| CK1δ | CST | 12417 | WB: 1:1,000 | 1D | 12 |
| Cyclin A2 | CST | 4656 | WB: 1:1,000 | 1I | This study |
| Cyclin B1 | CST | 4138 | WB: 1:1,000 | 1D, E and I, and 4A–D | This study |
| DYNLL1 | Abcam | EP1660Y | WB: 1:1,000 | 1D | This study |
| FAM83D (C‐term) | DSTT (University of Dundee) | Sheep SA102, third bleed | WB: 1:1,000; IF: 1:500 | 1A, D, E, H and I, and 2D and F, and 3B, C and F and 4A–D and 5B, C and F, and EV3A and B, and EV4B and EV5B | This study |
| FAM83D (N‐term) | DSTT (University of Dundee) | Sheep SA102, third bleed | WB: 1:1,000 | 1A and G, and 4G | This study |
| FAM83G | DSTT (University of Dundee) | Sheep S876C, third bleed | WB: 1:1,000 | EV4C | 12 14 |
| GAPDH | CST | 14C10 | WB: 1:5,000 | 1A, D, E, G, H and I, and 2D and F, and 4A–D and G, and 5B, C and F, and EV3A and B, and EV4A | |
| GAPDH | Proteintech | 10494‐01‐AP | WB: 1:5,000 | Appendix Fig S1A | |
| GFP | Roche | 11814460001 | WB: 1:500 | 3B | This study |
| HMMR | Millipore | ABC323 | WB: 1:1,000; IF: 1:500 | 1D, E and I, and 2D and 4B–D and EV2B and EV3A and B | This study |
| HMMR | Abcam | 124729 | WB: 1:1,000; IF: 1:500 | 4G and H | This study |
| p‐Histone H3 (S10) | CST | 9701 | WB: 1:1,000 | 4B | This study |
| VHL | CST | 68547 | WB: 1:1,000 | 2F | 22 23 |
CST, cell signaling technology; DSTT, division of signal transduction therapy; WB, Western blotting; IF, immunofluorescence.
All the antibodies used in this study are listed, along with their source, catalogue number and dilution factor used. References for antibody specificity and details of the figures in which each antibody was used are also provided.