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. 2019 Jul 24;20(9):e47250. doi: 10.15252/embr.201847250

Figure 2. R loop accumulation after depletion of the selected DNA Damage Checkpoint (DDC) and Post‐replicative Repair (PRR) candidates.

Figure 2

  1. Relative DRIP–qPCR signal values at RPL13A, APOE, MIB2, and RHOT2 genes in HeLa cells transfected with the indicated siRNAs and treated in vitro with RNase H pre‐immunoprecipitation where indicated. The mean ± SEM from at least three independent experiments is shown. *P < 0.05, **P < 0.01, ***P < 0.001 (one‐tailed paired t‐test).
  2. Representative images of immunostaining with S9.6 and anti‐nucleolin antibodies in HeLa cells transfected with the indicated siRNAs.
  3. Relative S9.6 signal intensity per nucleus after nucleolus signal removal in HeLa cells after cytoplasm pre‐extraction (CE) and treated in vitro with RNase III and RNase H where indicated. More than 500 total cells from three independent experiments were considered. The median of each population is shown. Boxes and whiskers indicate 25–75 and 10–90 percentiles, respectively. ***P < 0.001 (Mann–Whitney U‐test).
Data information: Black stars denote significant increases, whereas red stars denote significant decreases.