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. 2019 Jul 24;20(9):e47250. doi: 10.15252/embr.201847250

Figure 3. DNA–RNA hybrids are a source of DNA breaks in DDR‐deficient cells.

Figure 3

  1. Representative images of single‐cell alkaline gel electrophoresis (comet assay) of HeLa cells transfected with the indicated siRNAs and either pEGFP‐C1 (RNH1−) or pEGFP‐M27 (RNH1+).
  2. Comet tail moment from single‐cell alkaline gel electrophoresis (comet assay) of HeLa cells transfected with the indicated siRNAs and either pEGFP‐C1 (RNH1−) or pEGFP‐M27 (RNH1+). More than 250 total cells were considered. The mean ± SEM of the median from at least three independent experiments is shown, except for RAD1, RAD17, and UBE2B (n = 2). *P < 0.05, **P < 0.01, ***P < 0.001 (one‐tailed unpaired t‐test). Black stars denote significant increases, whereas red stars denote significant decreases.