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. 2019 Jul 30;20(9):e45907. doi: 10.15252/embr.201845907

Figure 6. PHF2 epigenetically regulates CREB‐driven transcription of target genes in hippocampal neurons.

Figure 6

DIV 14 neurons were transfected with siRNAs, then pretreated for 30 min with KN62 (10 μM), followed by treatment with NMDA (10 μM) and glycine (2 μM).
  • A
    Samples were collected 3 min after stimulation with NMDA and glycine, and the expressions of p‐CREB, CREB, and PHF2 were examined by immunoblotting.
  • B
    The mRNA levels of memory formation‐related genes were analyzed using RT–qPCR normalized to 18S rRNA levels.
  • C–E
    ChIP analysis represented the binding of PHF2 (C), CREB (D), and H3K9me2 (E) to Bdnf and Nr2b promoters in neurons.
  • F
    ChIP‐ChIP analysis showed that PHF2 and CREB are recruited together to the same region of Bdnf and Nr2b promoters.
Data information: In (A–F), data are representative of three independent experiments. In (B–F), data are presented as the mean ± SD (n = 3). ***P < 0.001, **P < 0.01, and *P < 0.05 (unpaired, two‐sided Student's t‐test).Source data are available online for this figure.