Fig. 4.
TRPC6 expression increases with age. Platelet extracts (2 × 108/ml) from Trpc6+/+ mice that are 4, 6 or 10 weeks-old were prepared, and the TRPC6 protein (and β-Actin as loading control) was probed using Western Blot. (A) Quantification of protein expression determined via Western blot; ***P < 0.001; ****P < 0.001, t-test. (B) Western blot data showing expression of TRPC6 in an age-dependent manner, along with β-Actin as loading control. This experiment was repeated 3 times, with blood pooled from three groups of 5–6 mice, at the specified age.