Figure 1.

Se Deficiency Induces TNNT2 Suppression and Ca²⁺ Overload in the Chicken Heart

(A) The mRNA and protein levels of TNNT2 in the chicken heart. The results were calculated from at least three independent experiments (n = 3). The data are expressed as the mean ± SD. (B) The mRNA and protein levels of TNNT2 in the cardiomyocytes. (C) The mRNA levels of CANA1S, NCX, CASR, CALM, Orai1, TRPC 1, TRPC 3, SERCA, PMCA, RyR 1, RyR 2, RyR 3, and STIM and the protein levels of NCX, CASR, CALM SERCA, and PMCA were detected in Se-deficient cardiomyocytes. (D) Cardiomyocytes were measured by the fluorescence intensity of Fluo-4 AM (green fluorescence, 5 mM). Cardiomyocytes were visualized using fluorescence microscopy; the greener the fluorescence, the greater was the concentration of Ca²⁺. (E) Cardiomyocytes were measured by a fluorescent microplate reader. Asterisks indicate significant difference from the corresponding normal (*p < 0.05). Control, normal cardiomyocytes; Control^vivo, control myocardial tissue; Se−, Se-deficient cardiomyocytes; Se−^vivo, Se-deficient myocardial tissue.