Figure 6.

lnc-3215 Was Involved in the Regulation of Ca²⁺ Homeostasis

(A) lncRNA levels of lnc-3215 were detected in the chicken heart. Control^vivo indicates control myocardial tissue. The results were calculated from at least three independent experiments (n = 3). The data are expressed as the mean ± SD. (B) The LncRNA levels of lnc-3215 were detected in Se-deficient cardiomyocytes. (C) The mRNA levels of CANA1S, NCX, CASR, CALM, Orai1, TRPC 1, TRPC 3, SERCA, PMCA RyR 1, RyR 2, RyR 3, and STIM and the protein levels of NCX, CASR, CALM SERCA, and PMCA were detected in the chicken embryo heart. Control^vivo indicates the normal chicken embryo heart. (D) The mRNA levels of CANA1S, NCX, CASR, CALM, Orai1, TRPC 1, TRPC 3, SERCA, PMCA, RyR 1, RyR 2, RyR 3, and STIM and the protein levels of NCX, CASR, CALM SERCA, and PMCA were detected in cardiomyocytes. Bars that do not share the same letters are significantly different (p < 0.05) from each other. (E and F) Cardiomyocytes were measured by the fluorescence intensity of Fluo-4 AM (green fluorescence, 5 mM). Cardiomyocytes were visualized using fluorescence microscopy and the fluorescent microplate reader; the greener the fluorescence, the greater was the concentration of Ca²⁺. Asterisk indicates significant difference from the corresponding control (*p < 0.05). Control, normal cardiomyocytes; Se−, Se-deficient cardiomyocytes; Se−^vivo, Se-deficient myocardial tissue; Si-lnc-3215, cardiomyocytes that transfect with Si-lnc-3215; Si-lnc-3215^vivo, chicken embryo heart that transfects with Si-lnc-3215.