Figure 3.

PCR-RFLP analysis of Leishmania species with the restriction enzyme NlaIII. Samples were run alongside a 50-bp molecular weight marker. Comparison of the band patterns of the DNA fragments generated from the NlaIII digestion of in silico, culture, and clinical isolates. Digestion with NlaIII can successfully differentiate between the extremely closely related Leishmania (Viannia) (L. [V.]) braziliensis and L. (V.) guyanensis/L. (V.) panamensis complex of the Viannia subgenus, which is extremely important in a clinical setting where treatment outcome can vary greatly between these two species.