Table 2.

Reaction conditions for each restriction enzyme used for the ND7 PCR-RFLP

Enzyme (µL)	Product source	Recognition site	Reaction buffer (µL)	Reaction conditions
HpyCH4IV (1 μL)	Helicobacter pylori CH4	5′… A/C G T …3′.3′… T G C/A …5′	1 × CutSmart® Buffer (2.5 μL)	2 hours at 37°C
NlaIII (1 μL)	Neisseria lactamica	5′… C A T G/ …3′ 3′… /G T A C …5′	1 × CutSmart Buffer (2.5 μL)	2 hours at 37°C