Figure 1.

EPS8 is overexpressed in multiple myeloma. (A) EPS8 mRNA expression in normal plasma cells from 4 volunteers (Normal) and MM cells from 23 MM patients or (B) 4 MM cell lines. CD138⁺ cells enriched from bone marrow by immunomagnetic cell sorting method (MACS). EPS8 mRNA in the cells was measured by quantitative real-time PCR. (C) Whole cell lysates were extracted from MM cell lines, MM patient samples and healthy donor samples and subjected to immunoblot analysis with the indicated antibodies. (D and E) EPS8 expression was analyzed by real-time PCR and immunoblotting in EPS8 knockdown (sh) and control cells (NC).