Figure 2.

EPS8 confers MM cell growth in vitro and in vivo. A. Cell viability was determined by CCK-8 assay, and viability of shRNA-transfected myeloma cells decreased in a time-dependent manner. B. Colony formation of myeloma cells in methylcellulose was performed according to the manufacturer’s instructions. Number of colonies was counted by microscopy on the 14^th day. C. CyclinD1, P21, p-Akt, Akt, p-Erk and Erk expression were analyzed by immunoblotting. GAPDH served as the loading control for each membrane. D. Cell cycle was analyzed by flow cytometry. E and F. The tumors excised from NSG mice after being inoculated subcutaneously with 8226/NC and 8226/sh cells for 28 days. Tumor weight was measured on the 28^th day.