Figure 6.

Menin inhibitor repressed HOXA9 gene expression by reducing H3K4me3 in the HOXA9 locus, which was rescued by MG132. MV4;11 and THP-1 cells treated for 10 h, with either control DMSO or 3 μM MI-503 +/- 10 μM MG132. A. HOXA9 mRNA level was detected by qRT-PCR. B. Schematic diagram to show two amplicons in HOXA9 promoter. C-F. ChIP assays were performed to look for menin, total H3, and H3K4m3 at amplicon 1 and 2 of the HOXA9 promoter (n=3). *P<0.05, **P<0.01, ***P<0.001. G. A molecular model for menin inhibitor-induced menin degradation and repression of HOX gene transcription.