Figure 1.

NK cells were enriched from splenocytes by magic bead-based sorting and the purity of NK cells (CD3ε^- CD49b⁺) was analyzed by flow cytometry (A). Gating strategy of flow cytometry of in vitro cytotoxic assay (B). Flow cytometric analysis of apoptosis in KPC cells and Pan02 cells treated with LNK cells or spleen NK cells in vitro (C), and KPC cell death at time 0 was shown in left panel. KPC cells were respectively cultured with spleen NK cells and LNK cells. Culture supernatants were harvested at 24 hours and analyzed for IFN-γ by Enzyme-linked immunosorbent assay (ELISA) (P<0.001) (D). After the last treatment, the serum of KPC tumor-bearing mice were collected to analyzed for INF-γ by ELISA (E).