Figure 4.

FBP1 was a downstream target gene of HSF2. A. Silencing HSF2 expression increased FBP1 expression in mRNA levels. B. Knockdown of HSF2 increased FBP1 protein levels in Huh7 and SMMC7721 cells. C. HSF2 suppressed FBP1 promoter activity in a dose-dependent manner. D. We mutated HSE on FBP1 promoter from TTCTGAACTTC to TTCTGTTCTTC to generate the HSE^Mut construct and luciferase assay results demonstrated that HSF2 lost its suppressive impact of HSE^Mut. E. HSF2 occupied the HSE in FBP1 promoter region. F. Patients with low levels of FBP1 expression have worse prognosis. G. HSF2 negatively correlated with FBP1 expression in TCGA-LIHC cohort.