Figure 4.

Reduction of GCN5 protein represses cell proliferation, increases the percentage of necrotic cells, and decreases c-MYC protein expression. A. Proliferation of murine ED1 and human H1355 NSCLC cell lines with stable knockdown of GCN5 by two independent shRNAs was monitored over 4 days. Proliferation rate was normalized to day 1. Representative immunoblots confirming GCN5 knockdown are shown in panel 3C. B. Apoptosis assays were performed in the murine ED1 NSCLC cell line with stable knockdown of GCN5 by shRNAs 3 days after seeding cells. Percentage of live, apoptotic and necrotic cells are shown. C. Stable reduction of endogenous GCN5 expression by two independent shRNAs introduced into murine and human lung cancer cells decreased endogenous c-MYC protein. Immunoblotting was performed using anti-GCN5 and anti-c-MYC antibodies. β-Actin served as a loading control. Symbols refer to *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.