Figure 7.

Effects of PDI inhibitors and free thiol blockers on the collagen gel contraction by MCF-10A, MCF-7 and MDA-MB-231 cells. Data presented as mean + SD, mini-max ranges marked as whiskers; n=12. NIE SEM! Collagen floating gels supplemented with the MCF-10A, MCF-7 and MDA-MB-231 cells in the NaOH-buffered medium containing DMEM, collagen type I, glutamine and antibiotics, were allowed to pomymerize (A) Two hours after polymerization gels were supplemented with PDI inhibitors and free thiol blockers, pictured and incubated for up to 72 hr (B) Significance of differences was analysed with one-way ANOVA and the post-hoc multiple comparisons Tukey’s test and planned comparisons were verified the bootstrap-boosted unpaired student’s t test (10000 iterations) with the Bonferroni’s correction for multiple comparisons.