Figure 2.

hCCR5Δ24 is not expressed at the cell surface of CD4⁺ T cells in PBMCs.(A and B). CCR5 surface and intracellular expression was measured by flow cytometry in human CD4⁺ T cells from PBMCs transiently transfected with pCMV5/HA‐wtCCR5 and pCMV5/HA‐hCCR5Δ24 using anti‐CCR5 2D7 (ECL 2) and anti‐CCR5 T21/8 (N‐term) mAbs (A) and anti‐HA mAbs (C). Statistical analyses of the flow cytometry experiments from (A). Statistical significance was considered when p ≤ 0.05 (**p ≤ 0.01, *p ≤ 0.05; N = 3 independent experiments). Error bars denote mean ± SD.