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. 2019 Sep 4;22(9):e25384. doi: 10.1002/jia2.25384

Figure 4.

Figure 4

The hCCR5Δ24 mutation increases CCR5 colocalization with Endoplasmic Reticulum but not Golgi markers. HEK 293T and HeLa‐CD4 cells were transiently transfected with pCMV5/HA‐wtCCR5 and pCMV5/HA‐hCCR5Δ24. Representative confocal micrographs of CCR5, ER (A) and Golgi (C) staining using anti‐HA, anti‐PDI and anti‐58K mAbs respectively. Pearson's colocalization correlation coefficients between anti‐HA and anti‐PDI (B) or anti‐58k (D) staining were calculated for regions of interest (ROI) corresponding to anti‐HA positive cells using Colocalization Threshold (Image J). Statistical significance was considered when p ≤ 0.05, **p ≤ 0.01, *p ≤ 0.05; N = 3 independent experiments). Error bars denote mean ± SD. 10 um scale.