Figure EV2. Effects of succinate on muscle fiber and mitochondrial function of C2C12 cells (related to Fig 5).

C2C12 cells were treated with 0, 0.5 mM, or 2 mM SUC for 48 h.

• A
  The mRNA expression of MyHC I, MyHC IIa, PGC‐1α, myoglobin, TnnT1 MyHC IIb, MyHC IIx, and TnnT3 in C2C12 cells.
• B, C
  Fluorescence‐activated cell sorting (FACS) analysis of TMRM fluorescence intensity and the relative mean fluorescence intensity of TMRM.

Data information: Results are presented as mean ± SEM (n = 5–6). Different letters between bars mean P ≤ 0.05 in one‐way ANOVA analyses followed by post hoc Tukey's tests.