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. 2019 Jul 18;20(9):e47892. doi: 10.15252/embr.201947892

Figure EV3. Role of SUNCR1/PLC‐β in succinate‐induced in vitro fiber‐type transition in myotubes (related to Fig 6).

Figure EV3

  • A
    [Ca2+]i of C2C12 cells treated with vehicle, SUC (2 mM), SUC (2 mM) + PLC‐β inhibitor U73122 (1 μM), or SUC (2 mM) + PLC‐β inhibitor U73122 (10 μM; n = 9–10).
  • B–D
    After 6 days of differentiation, C2C12 cells were treated with vehicle, SUC (2 mM), U73122 (5 μM), or SUC (2 mM) + U73122 (5 μM) for 48 hrs. Representative images (C) and (D, E) quantification of MyHC I and MyHC IIb immunofluorescent staining (green) in the C2C12 cells (n = 3). Scale bar in (C) represents 50 μm.
  • E
    C2C12 cells were transfected with vector or siSUNCR1, cultured for 6 days in a differentiation medium, and then treated with SUC (2 mM) for 48 h to test the concentration of lactic acid in medium (n = 5–6).
Data information: Results are presented as mean ± SEM. *P ≤ 0.05 by non‐paired Student's t‐test.