Figure EV4. SUNCR1 global knockout blocks the effect of succinate on protein synthesis (related to Fig 7).

• A
  Schematic representation of SUNCR1 KO by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) strategy. The sgRNA sites were located in intron 1 and intron 2 of SUNCR1 gene. Four sgRNAs were designed to delete exon 2 of SUNCR1 gene. The DNA sequences contained sgRNA‐binding regions are labeled with lines.
• B
  Immunoblots of SUNCR1 protein in liver, fat, soleus (sol), and gastrocnemius (gas) from WT and SUNCR1 KO mice.
• C
  Representative images for genotyping screen of WT, heterozygous (Het), and homozygous (KO) SUNCR1 KO mice.
• D–G
  (D) Cumulative food intake, (E) lean mass, (F) fat mass, and (G) body weight gain of WT or SUNCR1 KO mice after 6 weeks of dietary supplementation of 0 or 1% SUC.
• H, I
  Immunoblots and quantification of p‐mTOR, mTOR, p‐FoxO3a, FoxO3a, p‐AKT, and AKT proteins in gastrocnemius from WT or SUNCR1 KO mice after 6 weeks of dietary supplementation of 0 or 1% SUC (n = 3).

Data information: Results are presented as mean ± SEM (n = 5–6). *P ≤ 0.05 by non‐paired Student's t‐test.