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. 2019 Jul 18;20(9):e47892. doi: 10.15252/embr.201947892

Figure 5. Effects of succinate on MyHC expression, mitochondria biosynthesis, and metabolism in C2C12 cells.

Figure 5

C2C12 cells were treated with 0, 0.5, and 2 mM SUC for 48 h.
  • A, B
    Representative images and quantification of MyHC I and MyHC IIb immunofluorescent staining (green) in C2C12 cells (n = 16).
  • C–E
    The enzymes activity of (C) SDH, (D) LDH, and (E) lactate production in C2C12 cells.
  • F
    Quantification of mitochondrial DNA contents in C2C12 cells.
  • G–J
    (G) Mitochondrial electron microscopy showed the (H) mitochondrial density, (I) mitochondrial coverage, and (J) average mitochondrial area in C2C12 cell. Scale bar in (A) represents 50 μm; scale bar in (G) represents 0.5 μm.
Data information: Results are presented as mean ± SEM (n = 6–8). Different letters between bars mean P ≤ 0.05 in one‐way ANOVA analyses followed by post hoc Tukey's tests. *P ≤ 0.05 by non‐paired Student's t‐test.