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. 2019 Jul 18;20(9):e47892. doi: 10.15252/embr.201947892

Figure 8. Gastrocnemius‐specific SUNCR1 knockdown abolishes the effect of succinate on muscle fiber switch in vivo .

Figure 8

Male C57BL/6J mice were injected with LV‐shScrambled or shSUNCR1 lentivirus specifically into the gastrocnemius at 6 weeks of age. After 2 weeks of recovery, mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks.
  • A
    Timeline of the experimental protocol.
  • B
    SUNCR1 protein expression in gastrocnemius from mice transfected with shSUNCR1 lentivirus or LV‐shScrambled (n = 3).
  • C–E
    (C) The running time in low speed, (D) four‐limb handing time, and (E) muscle grip strength of both control and gastrocnemius‐specific SUNCR1 knockdown mice.
  • F–H
    The enzymes activity of (F) HK, (G) LDH, and (H) SDH in gastrocnemius.
  • I, J
    Immunoblots and quantification of MyHC I, MyHC IIb, NFAT, and PGC‐1α protein in gastrocnemius (n = 3). Data information: Results are presented as mean ± SEM (n = 5–8). *P ≤ 0.05 by non‐paired Student's t‐test.