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. 2019 Jul 29;20(9):e48235. doi: 10.15252/embr.201948235

Figure 6. IRF2 controls caspase‐4 levels in human induced pluripotent stem cell (iPSC)‐derived macrophages.

Figure 6

  • A, B
    Macrophages were derived from human iPSC clones knock‐out for the indicated genes. Two clones were selected for IRF2 KO cells. (A) Caspase‐4, caspase‐1, IRF2, and β‐actin protein levels were assessed by Western blotting analysis. One experiment representative of two independent experiments is shown. (B) The indicated mRNA levels were quantified by qRT–PCR normalized to β‐actin mRNA levels and expressed as fold change compared to WT levels. Each dot represents the average of a technical RT–PCR triplicate from one experiment; the bar represents the mean of three independent experiments. One‐way ANOVA with Dunnet's multiple comparisons test was performed (WT vs. IRF2 KO #I17 **P = 0.0020; WT vs. IRF2 KO #32 **P = 0.0013 for CASP4 transcript. WT vs. CASP1 KO ***P < 0.0001 for CASP1 transcript levels).

Source data are available online for this figure.