Figure 5. Representative images of the two-dimensional electrophoresis fluorescence difference (DIGE) gels used for comparative analysis. Pooled protein extracts from the tumor and control samples were labelled with Cye3 and Cye5, respectively. The internal control mix (Cye2-labeled), used for normalization, was formed by mixing equal amounts of protein from the tumor and the control pools. The superimposed image was the product of superimposing all three Cye dye images; images were pseudo-coloured.
