Figure 3. Validation of candidate protein abundance by western blot analysis. A and B: The average fold change (FC) in protein abundance in coculture (CC) compared to control (Con) MCF-7 and MDA-MB-231 cells, for each candidate protein (NDRG1, PGK1 and TFF1) as measured by iTRAQ LC-MS/MS (white) and western analysis (grey). Data is presented as mean±SEM. C and D: Representative western blots for the abundance measurement of PGK1, TFF1 and NDRG1 in MCF-7 (Con), CAA-MCF-7 (CC), MDA-MB-231 (Con) and CAA-MDA-MB-231 (CC).