Skip to main content
. 2018 Apr 16;39(9):1836–1848. doi: 10.1177/0271678X18769770

Figure 2.

Figure 2.

Thorase elicits neuroprotection. (a) Immunoblot of Thorase expression in primary cultured cortical neurons after transfection with lentivirus expressing EGFP (LV.cFUGW), wild type Thorase (LV.Thorase) or mutant Thorase (LV. mAB) with both mutated Walker A and Walker B domains. Control cultures were not transfected. Immunoblot is representative of three independent experiments. (b) Cell viability assessed by PI/Hoechst staining in primary cortical cultures transfected with LV.cFUGW, LV.Thorase or LV. mAB following exposure to OGD for 90 min. Scale bar, 25 μM. (c) Percentages of neuron death were quantified and expressed as the mean ± SEM from three independent experiments performed in triplicate (n = 9). *p < 0.05 by ANOVA with Tukey–Kramer post hoc test compared to LV.cFUGW viral control. (d) Cell viability assessed in transfected primary cortical cultures after exposure to 500 µM NMDA (5 min) by PI/Hoechst staining. Scale bar, 25 μM. (e) Percentages of neuron death were quantified and expressed as the mean ± SEM from three independent experiments performed in triplicate (n = 9). *p < 0.05 by ANOVA with Tukey–Kramer post hoc test compared to LV.cFUGW viral control.