Figure 1.

T_reg magnetic cell isolation procedure. Whole spleens were isolated from mice and manually dissociated into a single-cell solution to remove extracellular matrix (ECM). The solution was passed through a strainer and then incubated with magnetic bead conjugated antibodies. Labeled cells were then passed through a magnetic field with metal beads. Non-CD4⁺ cells were depleted from the solution and CD4⁺/CD25⁺ cells were sorted from the remaining solution to yield CD4⁺/CD25⁺ T_regs.

ECM: extracellular matrix.