Figure 4.
Increasing the ratios of Tregs in BMSC treatment after OGD/R decreases neuroprotection capacity of Tregs. (a) Treatment with BMSCs and the native ratio of Tregs confers significant neuroprotection after OGD/R. Supplemental Tregs in ratios of 1:10 and 1:1 Tregs: BMSCs show significant reduction in neuroprotection relative the native 1:100 ratio. (b) FGF-β, a cytokine secreted by BMSCs promoting proliferation and differentiation, was measured by ELISA. Increasing the ratio of Tregs correlates to a dose-dependent decrease in FGF-β secretion. (c) BMSC IL-6 secretion was measured by ELISA. IL-6 production was significantly increased in the 1:10 Treg ratio treatment group, while it was significantly decreased in the 1:1 ratio treatment group.
OGD: oxygen-glucose deprivation; IL-6: interleukin-6: FGF-β: fibroblast growth factor-beta.