Figure 6.

Astrocyte activation after hypothermia was measured using markers for GFAP, LNC2, and Serpin-A3N. Panel (a) shows representative pictomicrographs illustrating astrocyte activation using GFAP (green), LNC2 (red) and SERPIN-A3N (magenta) markers. Increasing durations of hypothermia suppressed astrocyte expression of GFAP and Serpin-A3N, two markers that are associated with the protective astrocyte phenotype. The marker LNC2, which is associated with the toxic astrocyte activation phenotype, was not suppressed by 6 or 2 h TH. The suppression was dose-dependent, in that 33 ºC suppressed more than 35 ºC which suppressed more than 37 ºC. Scale bar = 30 μm. Panel (b) shows the data normalized to cultures maintained at normal oxygen and glucose (No OGD) in order to emphasize the relative change.