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. 2018 Apr 6;39(9):1776–1789. doi: 10.1177/0271678X18769188

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Figure 4. — EphrinB2 knockdown and angiogenesis in the ipsilateral thalamus after cortical infarction. (a) Immunostaining for BrdU and laminin in the ipsilateral thalamus of the Scramble-shRNAand Efnb2-shRNA groups at seven days after MCAO. Efnb2-shRNA treatment markedly decreased BrdU⁺ cells (green) that co-stained for laminin (red). Scale bar: 50 µm. (b) Laminin-labeled blood vessel significantly decreased in the ipsilateral thalamus of the Efnb2-shRNA group at seven days after MCAO. (c and d) Quantitative analysis of the number of BrdU⁺/laminin⁺ cells and density of blood vessel. n = 8, data are expressed as median ± interquartile range. *P < 0.05 compared with the Scramble-shRNA group.