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. 2019 Aug 29;10:1942. doi: 10.3389/fimmu.2019.01942

Figure 2.

Figure 2

Treg cell ablation results in advanced tumor staging. (A,B) 10-week-old mammary gland whole mount representative images (A) and quantification (B) of the percentage of tumor area of control (black) and Treg cell-ablated (green) mice (n = 16–17 individual mammary glands). Scale bars represent 5 mm, *p = 0.0405 calculated by Mann-Whitney test. (C,D) Representative image (C) and quantification (D) of tumor stage. Arrows indicate examples of various tumor stages: hyperplasia/adenoma (green arrow), early invasive carcinoma (black arrow), and late invasive carcinoma (white arrow) (E) α-SMA myoepithelial staining confirming disruption of myoepithelial layer (F,G), representative image (F) and quantification (G) of desmoplasia; (H,I) representative image of Masson trichome staining (H) and quantification (I) of collagen deposition; and (J,K) representative image (J) and quantification (K) of inflammatory infiltration. (E,F,H,J) White arrows indicate examples of histological observation. Values are expressed as mean ± SEM. Tumor staging was compared by two-way ANOVA, ****p < 0.0001; other comparisons were done by Mann-Whitney test. ****p < 0.0001 and **p = 0.0021. Data were pooled from four independent experiments. (C,E,F,H,J) Scale bars represent 250 μm.