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. 2019 Aug 29;10:940. doi: 10.3389/fphar.2019.00940

Figure 2.

Figure 2

Overexpression of miR-532-3p inhibits cell proliferation and facilitates apoptosis in TSCC. (A) Expression of miR-532-3p was detected via real-time PCR in four types of TC cells, TSCCA, TCA8113, CAL-27, and SCC-25. Relatively highest and lowest miR-532-3p expression were in TCA8113 and CAL-27 cells, respectively. (B) Expression of miR-532-3p was increased after transfection miR-532-3p mimics in CAL-27 cell. When TCA8113 was transfected with miR-532-3p inhibitor, the expression of miR-532-3p was decreased. Cell proliferation was measured using CCk-8 assay (C), Ki-67 protein expression analysis (D) and cell cycle detection (E, F). (G) Promotion of apoptosis by overexpression of miR-532-3p. TCA8113 and CAL-27 cells were transfected for 48 h and apoptotic cells were monitored with flow cytometry after Annexin V and PI staining (H). Apoptosis-related proteins (caspase-3 and PARP) were tested by western blot. **p < 0.01 vs. miR-532-3p mimics; #p < 0.05, ##p < 0.01 vs. miR-532-3p inhibitor.