Figure 3.

Cpt1a levels were reduced in CSE-treated mouse PMVECs and in PMVECs from COPD patients. (A) Mouse PMVECs were treated with CSE (0.25%, 12 h), RNA was extracted, and Cpt1a, Cpt1b, and Cpt1c mRNAs were measured by qRT-PCR. (B) Levels of Cpt1a protein were measured in mouse PMVECs treated with CSE by Western blot. (C) Cpt1a mRNA was measured by qRT-PCR in PMVECs from healthy subjects and COPD patients. (D) Levels of Cpt1a protein were measured in human PMVECs by Western blot. Data are expressed as mean ± SEM, n = 5–6. ***P < 0.001 vs. control (Ctr) or healthy group.