Figure 2: Effect of drug administration sequence on proliferation inhibition in non-concurrent drug treatment.

Cells were seeded and treated with individual drugs for 72 hr, washed with DPBS, and treated with the second drug for an additional 72 hr. Drug concentrations were chosen as multiples of approximate IC₅₀s for colony formation. A. U1242 cells were treated with alisertib, carboplatin, alisertib followed by carboplatin or carboplatin followed by alisertib. B. U87 cells were treated with alisertib, irinotecan, alisertib followed by irinotecan or irinotecan followed by alisertib. All experiments were performed twice. Average percent survivals of both experiments are shown.