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. Author manuscript; available in PMC: 2019 Sep 5.
Published in final edited form as: Cell Rep. 2019 Aug 27;28(9):2288–2292.e3. doi: 10.1016/j.celrep.2019.07.089

Figure 2. Detection and Sequencing of tyr Crispants.

Figure 2.

(A) Albino tyr crispant (left) and wild-type (right) age-matched hatchlings.

(B) PCR primer placement (P1–P4) relative to CRISPR target sites A (blue), B (pink), and C (cyan). Representative PAGE results are shown for seven of the mutant lizards. Colored arrows denote bands with altered mobility relative to wild type (WT).

(C) Sequences of CRISPR-Cas9-induced indels from representative tyr crispants. (Top) Mut1 and Mut4–7 sequences with deletions. (Bottom) Mut2 and Mut3 sequences with insertions. Targeted guide sites A (blue), B (pink), and C (cyan) are highlighted in WT reference sequences. tyr crispants deletions are indicated in bold, insertions boxed, and sequences matching WT in gray.

See also Figure S2.